Recombinant HLA-A2 抗体
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Quick Overview for Recombinant HLA-A2 抗体 (ABIN7543207)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-HLA-A2 [BB7.2], Rabbit IgG, kappa
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特异性
- This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.
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亚型
- IgG kappa
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anti-HLA-A2 antibody
适用: 人 FACS, IP 宿主: 小鼠 Monoclonal BB7-2 unconjugated
anti-HLA-A2 antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal BB7-2 PE
anti-HLA-A2 antibody适用: 人 FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal BB7-2 unconjugated
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应用备注
- The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 Molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- HLA-A2
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物质类
- Influenza Protein
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背景
- HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A-2 HLA-A, HLA class I histocompatibility antigen A-28 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A-28
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UniProt
- P04439
抗原
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