Recombinant TPBG 抗体
Quick Overview for Recombinant TPBG 抗体 (ABIN7543175)
抗原
See all TPBG 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-5T4 [5T4 (H8)], Rabbit IgG, kappa
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特异性
- This antibody binds 5T4 antigen expressed on various carcinomas. It is reported that epitope recognized by this antibody is present in the extracellular domain of the molecule more particularly towards the membrane proximal leucine rich repeat 2 (LRR2) (PMID: 11903056).
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing male BALB/c mice with wheat germ agglutinin (WGA) purified glycoproteins from deoxycholate (DOC) solubilised human syncytiotrophoblast plasma membrane (StMPM).
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亚型
- IgG kappa
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anti-Trophoblast Glycoprotein (TPBG) (AA 219-328) antibody
TPBG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B6 unconjugated
anti-Trophoblast Glycoprotein (TPBG) (AA 32-351) antibodyTPBG 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Trophoblast Glycoprotein (TPBG) (AA 34-356) antibodyTPBG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Trophoblast Glycoprotein (TPBG) antibodyTPBG 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 6E9 unconjugated Recombinant Antibody
anti-Trophoblast Glycoprotein (TPBG) (AA 1-420) antibodyTPBG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Trophoblast Glycoprotein (TPBG) (AA 32-355) antibodyTPBG 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM137 unconjugated
anti-Trophoblast Glycoprotein (TPBG) antibodyTPBG 适用: 人 ELISA, IHC 宿主: 兔 Monoclonal 1D4 unconjugated Recombinant Antibody
anti-Trophoblast Glycoprotein (TPBG) (AA 219-328) antibodyTPBG 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Trophoblast Glycoprotein (TPBG) (N-Term) antibodyTPBG 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Trophoblast Glycoprotein (TPBG) (AA 1-420) antibodyTPBG 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- This antibody binds 5T4 antigen with a very high affinity of K(D)=1.8x10(-11) M (PMID: 11113573). The reactivity of this antibody to solubilised human syncytiotrophoblast plasma membrane (StMPM) was checked using immunodotting on nitrocellulose membrane and ELISA. The distribution of the 5T4 antigen was determined using mAb 5T4 by indirect immunoperoxidase staining of sections of normal organ and placental tissues as well as immunofluorescence and radiobinding assays with a wide variety of cell lines representing differing normal and tumour cell types. Immunoprecipitation of radiolabelled StMPM using this antibody indicated that 5T4 Molecules are glycoproteins of mol. wt of approximately 72 kD on SDS-PAGE (PMID: 3355761). This paper (PMID: 2404511) studies the immunohistochemical distribution of 5T4 antigen and reports that 54T4 antigen is present in a wide range of tumor cells including carcinomas of the bladder, breast, cervix, endometrium, lung, oesophagus, ovary, pancreas, stomach and testicular non-seminomatous germ cell tumours. 5T4 can serve as a marker for detection of tumor. Various papers report the use of this antibody for the study of expression of 5T4 antigen in various types of cancers (PMID: 2404512, 1419629, 8180020, 11578488).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- TPBG (Trophoblast Glycoprotein (TPBG))
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别名
- 5T4
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背景
- TPBG, M6P1, WAIF1, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncofetal trophoblast glycoprotein, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
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UniProt
- Q13641
抗原
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