Recombinant SARS-CoV-2 Nucleocapsid 抗体
Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid 抗体 (ABIN7543169)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7], Rabbit IgG, kappa
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特异性
- This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.
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交叉反应
- SARS Coronavirus (SARS-CoV)
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The original version of this antibody was raised against SARS-CoV-1/2.
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亚型
- IgG kappa
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应用备注
- The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS-CoV-2 (2020, pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10 % neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020, pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020, pmid: 32995783).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- SARS-CoV-2 Nucleocapsid
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物质类
- Viral Protein
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背景
- Nucleoprotein, NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV-2 Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV, 1C7, severe acute respiratory syndrome virus
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UniProt
- P0DTC9
抗原
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