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Recombinant SARS-CoV-2 Nucleocapsid 抗体

This 兔 Chimeric antibody specifically detects SARS-CoV-2 Nucleocapsid in ELISA, WB, FACS 和 IHC. It exhibits reactivity toward SARS Coronavirus-2 (SARS-CoV-2).
产品编号 ABIN7543169
发货至: 中国
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Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid 抗体 (ABIN7543169)

抗原

See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体
SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

抗体类型

Recombinant Antibody

适用

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SARS Coronavirus-2 (SARS-CoV-2)

宿主

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克隆类型

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Chimeric

标记

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This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

克隆位点

1C7C7
  • 原理

    Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7], Rabbit IgG, kappa

    特异性

    This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.

    交叉反应

    SARS Coronavirus (SARS-CoV)

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2a

    纯化方法

    Protein A affinity purified

    免疫原

    The original version of this antibody was raised against SARS-CoV-1/2.

    亚型

    IgG kappa
  • 应用备注

    The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS-CoV-2 (2020, pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10 % neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020, pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020, pmid: 32995783).

    说明

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

    别名

    SARS-CoV-2 Nucleocapsid

    物质类

    Viral Protein

    背景

    Nucleoprotein, NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV-2 Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV, 1C7, severe acute respiratory syndrome virus

    UniProt

    P0DTC9
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