Recombinant Disialoganglioside 2 (GD2) 抗体
This 兔 Chimeric antibody specifically detects Disialoganglioside 2 in FACS 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN7543163
发货至:
中国
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Quick Overview for Recombinant Disialoganglioside 2 (GD2) 抗体 (ABIN7543163)
抗原
Disialoganglioside 2 (GD2)
抗体类型
Recombinant Antibody
适用
人
宿主
兔
克隆类型
Chimeric
标记
非结合性
应用范围
Flow Cytometry (FACS), ELISA
克隆位点
14G2a
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原理
- Anti-Disialoganglioside 2 [14G2a], Rabbit IgG, kappa
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特异性
- This antibody binds the GD2 antigen and GD2 expressing neuroblastoma cell lines with an affinity similar to the parent antibody.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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纯化方法
- Protein A affinity purified
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免疫原
- The original murine parent antibody was generated against human neuroblastoma cell line LAN-1. This IgG2a isotype version was then generated by class switching of the murine parent antibody 14.18 (IgG3).
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亚型
- IgG kappa
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应用备注
- This antibody is a IgG2a isotype switch variant of the murine parent antibody 14.18. This antibody can direct efficient complement-dependent cytotoxicity against neuroblastoma tumor cells and melanoma cell lines in vitro in the presence of human complement (PMID: 2720646). 14G2a was coupled to the ribosome-inactivating plant toxin gelonin with the heterobifunctional cross-linking reagentN-succinimidyl-3(2-pyridyldithio)propionate. The immunoreactivity and specificity of 14G2a-gelonin immunotoxin determined by ELISA. Assays for complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC) revealed that these functional properties of the native 14G2a antibody were also preserved in the 14G2a-gelonin immunotoxin (PMID: 1756537). An immunotoxin prepared by linked 14G2a via the bivalent linker N-succinimidyloxycarbonyl-amethyl-a-(2-piridyldithio)toluene to deglycosylated ricin A chain was shown to inhibit the protein synthesis of neuroblastoma cell lines IMRS and NMB (Gottstein et al., 1994). Treatment with 14G2a mAb (1-4 mg/kg cumulative dose given as five i.v. daily administrations) dramatically reduced the metastatic spread of NB cells and prolonged the long-term survival of treated mice in a dose-dependent manner (PMID: 12880983). This antibody is also reported to be used in the study of interactions between peptides mimicking the GD2 ganglioside using flow ctyometry and ELISA (PMID: 21455557).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Disialoganglioside 2 (GD2)
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别名
- Disialoganglioside 2
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背景
- GD2
抗原
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