Recombinant T Cell Receptor 抗体
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- 抗原
- T Cell Receptor
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR)
- 原理
- Anti-TCR [BMA031], Rabbit IgG, kappa
- 特异性
- This antibody reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. TCR-αβ is expressed by the majority of mature CD3+ T cells.
- 产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
- 纯化方法
- Protein A affinity purified
- 免疫原
- The original antibody was generated by immunizing mice with human perihperal blood T-lymphocytes.
- 克隆位点
- BMA031
- 亚型
- IgG kappa
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anti-T Cell Receptor antibody
适用: 人 FACS, IP, IHC (fro), ELISA 宿主: 小鼠 Monoclonal 11F2 unconjugated
anti-T Cell Receptor antibody适用: 小鼠 FACS, IP, Crys, IF 宿主: 兔 Chimeric Desire-1 unconjugated Recombinant Antibody
anti-T Cell Receptor antibody适用: 小鼠 FACS, IHC 宿主: 仓鼠 Monoclonal GL-3 unconjugated
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- 应用备注
- This antibody is recomended for detection of viable human αβ+ TCR lymphocyte. It is highly effective in modulating immune reactions and is capable of activating human T lymphocytes in vito (PMID: 3092794). Double immunofluorescence staining was used to ascertain that this antibody does not cross react with γδ+ TCR cells. FACS analysis confirmed that BMA031 did not react with γδ+ TCR cells. BMA031 successfully precipitates TCR αβ/CDR complex (PMID: 1704361). BMA031 in conjugation with triple drug induction treatment in kidney-transplanted patients showed no side effects and reduction in graft rejections (PMID: 2978369). A clinical phase II trial administered 50 mg of murine anti-human alpha/beta-TCR mAb (BMA 031) intravenously on the day of, as well as 2 and 4 days after, cadaveric donor renal transplantation in combination with a CsA/prednisone regimen. After a minimum of 30 months follow-up, the actual allograft survival rate was 87 % in the BMA-treated group compared with 68 % in the control cohort (PMID: 8009591).Humanized version of this antibody BMA 031-EUCIV3 is found to have 2.5 times less affinity than its murine parent, however the ability to cause antibody dependent cell-mediated cytolysis was significantly enhanced (PMID: 1836485).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- T Cell Receptor
- 别名
- TCR
- 背景
- Human α/β T Cell Receptor (TCR)
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