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Recombinant CD43 抗体

This anti-CD43 antibody is a 小鼠 单克隆 antibody detecting CD43 in FACS, WB 和 RIA. Suitable for 大鼠.
产品编号 ABIN7543049
发货至: 中国

Quick Overview for Recombinant CD43 抗体 (ABIN7543049)

抗原

See all CD43 (SPN) 抗体
CD43 (SPN) (Sialophorin (SPN))

抗体类型

Recombinant Antibody

适用

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大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD43 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Radioimmunoassay (RIA)

克隆位点

OX-58
  • 原理

    Anti-CD43 [OX-58], Mouse IgG2b, kappa

    特异性

    This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility.

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2b

    纯化方法

    Protein A affinity purified

    免疫原

    This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.

    亚型

    IgG2b kappa
  • 应用备注

    This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    CD43 (SPN) (Sialophorin (SPN))

    别名

    CD43

    背景

    Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, W3/13 antigen, OX 58, MRC OX 58

    UniProt

    P13838

    途径

    Regulation of Leukocyte Mediated Immunity
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