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Recombinant CD53 抗体

This anti-CD53 antibody is a 兔 Chimeric antibody detecting CD53 in FACS, IP, IHC (fro) 和 IHC (p). Suitable for 大鼠.
产品编号 ABIN7543047
发货至: 中国

Quick Overview for Recombinant CD53 抗体 (ABIN7543047)

抗原

See all CD53 抗体
CD53

抗体类型

Recombinant Antibody

适用

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大鼠

宿主

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克隆类型

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Chimeric

标记

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This CD53 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

OX-44
  • 原理

    Anti-CD53 [OX-44], Rabbit IgG, kappa

    特异性

    This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is dependent on disulphide bonding.

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    纯化方法

    Protein A affinity purified

    免疫原

    This antibody was prepared by immunizing BALB/c mice with T lymphoblasts from an MLR between purified rat T helper cells and irradiated semiallogeneic rat spleen.

    亚型

    IgG kappa
  • 应用备注

    This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12 % of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-

    说明

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    CD53

    别名

    CD53

    背景

    Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Leukocyte antigen MRC OX-44

    UniProt

    P24485
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