Recombinant CA9 抗体
Quick Overview for Recombinant CA9 抗体 (ABIN7542959)
抗原
See all CA9 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Carbonic Anhydrase IX [M75], Mouse IgG1, kappa
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特异性
- This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.
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亚型
- IgG1 kappa
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anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibody
CA9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7C4A5 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 59-414) antibodyCA9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibodyCA9 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 10F7A8 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 52-151) antibodyCA9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 251-350) antibodyCA9 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 9B5 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IP 宿主: 小鼠 Monoclonal 12F10 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人, 马 WB, IHC, StM 宿主: 小鼠 Monoclonal CA9-781 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18383 unconjugated
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应用备注
- The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992, PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000, PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 μM (Král et al., 2008, PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009, PMID: 19538935).
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说明
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This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CA9 (Carbonic Anhydrase IX (CA9))
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别名
- Carbonic Anhydrase IX
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背景
- CA9, CAIX, MN/CA IX, endogenous protein p54/58N
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UniProt
- Q16790
抗原
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