SARS-CoV-2 Spike 抗体 (RBD)
(3 references)
Quick Overview for SARS-CoV-2 Spike 抗体 (RBD) (ABIN7540678)
抗原
See all SARS-CoV-2 Spike 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- RBD
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Supplier Product No.
- 600-401-ms8s
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Supplier
- Rockland
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原理
- SARS-CoV-2 Spike Protein (RBD) Antibody
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交叉反应 （详细）
- Anti-SARS-CoV-2 Spike affinity purified antibody is directed against SARS Coronavirus 2 Spike protein.
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纯化方法
- The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 Spike resin.
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过滤
- Sterile filtered
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).
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亚型
- IgG
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anti-SARS-CoV-2 Spike (C-Term) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta, SARS CoV-2 Gamma, SARS CoV-2 Alpha ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike (AA 319-541) antibodyAnimal-Free 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, FACS, IF 宿主: 小鼠 Monoclonal AB68-A09 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: Human Monoclonal AM004414 (414-4) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal AM015553 (553-15) unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (AA 319-541) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM54 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
anti-SARS-CoV-2 Spike antibodyResearch Grade 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike (RBD) antibodyVerified 适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-SARS-CoV-2 Spike (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, FACS 宿主: 兔 Monoclonal DM26 unconjugated
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应用备注
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ELISA_Dilution: 1:150,000 - 1:450,000
IF_Microscopy_Dilution: 1:1000
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说明
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Suggested Applications: Other
Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and IF. This antibody shows reactivity to recombinant SARS COV-2, S1 subunit protein and recombinant SARS COV-2 whole spike protein. Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).
: "Within-host evolution of SARS-CoV-2 in an immunosuppressed COVID-19 patient as a source of immune escape variants." in: Nature communications, Vol. 12, Issue 1, pp. 6405, (2021) (PubMed).
: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).
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: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).
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- SARS-CoV-2 Spike
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别名
- SARS-CoV-2 Spike Protein
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背景
- Rabbit anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2),SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.
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UniProt
- P0DTC2
抗原
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