SARS-CoV-2 Spike 抗体 (RBD)
Quick Overview for SARS-CoV-2 Spike 抗体 (RBD) (ABIN7540677)
抗原
See all SARS-CoV-2 Spike 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- RBD
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Supplier Product No.
- 600-401-ms8
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Supplier
- Rockland
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原理
- SARS-CoV-2 Spike Protein (RBD) Antibody
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交叉反应 (详细)
- Anti-SARS-CoV-2 Spike affinity purified antibody is directed against SARS Coronavirus 2 Spike protein.
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纯化方法
- The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 Spike resin.
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过滤
- Sterile filtered
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:150,000 - 1:450,000
IF_Microscopy_Dilution: 1:1000
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说明
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Suggested Applications: Other
Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and IF. This antibody shows reactivity to recombinant SARS COV-2, S1 subunit protein and recombinant SARS COV-2 whole spike protein. Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).
: "Within-host evolution of SARS-CoV-2 in an immunosuppressed COVID-19 patient as a source of immune escape variants." in: Nature communications, Vol. 12, Issue 1, pp. 6405, (2021) (PubMed).
: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).
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: "Antibody escape and global spread of SARS-CoV-2 lineage A.27." in: Nature communications, Vol. 13, Issue 1, pp. 1152, (2022) (PubMed).
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- SARS-CoV-2 Spike
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别名
- SARS-CoV-2 Spike Protein
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背景
- Rabbit anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, Spike glycoprotein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, S glycoprotein, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2),SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development. Anti-SARS-CoV-2 Spike Protein Antibody is useful for researchers interested in diagnostics, vaccines, and viral research.
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UniProt
- P0DTC2
抗原
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