SARS-CoV-2 NSP13 抗体 (AA 584-601)
(2 references)
Quick Overview for SARS-CoV-2 NSP13 抗体 (AA 584-601) (ABIN7540671)
抗原
See all SARS-CoV-2 NSP13 (HEL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 584-601
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Supplier Product No.
- 100-401-a54
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Supplier
- Rockland
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原理
- Sars-Cov Nonstructural Protein 13 Antibody
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交叉反应 （详细）
- This antibody is directed against SARS-Coronavirus nsp13 protein.
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纯化方法
- The product is neat antiserum.
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过滤
- Sterile filtered
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免疫原
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 584-601) of the SARS Coronavirus nonstructural protein 13.
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亚型
- IgG
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anti-SARS-CoV-2 Helicase (NSP13) (HEL) (C-Term) antibody
HEL 适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: 1:60
IF_Microscopy_Dilution: 1:800
Western_Blot_Dilution: 1:1,000
Other: IMMUNOELECTRON MICROSCOPY 1:300
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说明
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Suggested Applications: IF, Multiplex
This antibody is suitable for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: None
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).
: "Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase." in: Journal of virology, Vol. 78, Issue 11, pp. 5619-32, (2004) (PubMed).
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: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).
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- SARS-CoV-2 NSP13 (HEL) (SARS-CoV-2 Helicase (NSP13) (HEL))
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别名
- SARS-CoV-2 NSP13
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背景
- Rabbit anti-Sars-Cov Nonstructural Protein 13 Antibody, Replicase polyprotein 1a, pp1a antibody, Growth factor-like peptide,The coronavirus nonstructural protein 13 (nsp13) is one of the SARS-Coronavirus replicase cleaving products encoded by ORF1b. Nsp13 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp13 contains a C-terminal NTPase/Helicase domain and an N-terminal putative zinc-binding motif. Anti-SARS-CoV Nonstructural Protein 13 (nsp13) Antibody is useful for researchers interested in viral research.
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基因ID
- 1489680, 30124074
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UniProt
- P0C6U8
抗原
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