T7 RNA Polymerase 抗体
The 兔 多克隆 anti-T7 RNA Polymerase antibody is suitable to detect T7 RNA Polymerase in samples from Bacteriophage T7. It has been validated for ELISA 和 WB.
产品编号 ABIN7540668
Supplier Product No.: 200-401-mx5
发货至:
中国
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Contact Info
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Quick Overview for T7 RNA Polymerase 抗体 (ABIN7540668)
抗原
T7 RNA Polymerase
适用
Bacteriophage T7
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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Supplier Product No.
- 200-401-mx5
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Supplier
- Rockland
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原理
- T7 RNA Polymerase Antibody
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交叉反应 （详细）
- Protein A purified T7 RNA Polymerase antibody is directed against T7 Polymerase (E.coli)
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过滤
- Sterile filtered
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免疫原
- Anti-T7 Polymerase antibody was produced from whole rabbit serum produced by repeated immunizations with T7 Polymerase isolated from E. coli.
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:11,000-1:13,000
Western_Blot_Dilution: 1.0 μg/mL
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说明
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This protein A purified Anti-T7 RNA Polymerase antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 98.9 kDa in size corresponding to T7 Polymerase by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- T7 RNA Polymerase
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背景
- Escherichia phage T7 (Bacteriophage T7), DNA-directed RNA polymerase, DNA-dependent RNA polymerase, RNA nucleotidyltransferase (DNA-directed),T7 RNA Polymerase is a highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. It recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. T7 RNA Polymerase unwinds the double-stranded DNA to expose the coding strand for templating. It participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also, T7 RNA Polymerase plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process. In eukaryotes three forms of this enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized.
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基因ID
- 1261050
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NCBI登录号
- NP_041960
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UniProt
- P00573
抗原
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