Nuclease 抗体
This anti-Nuclease antibody is a 兔 多克隆 antibody detecting Nuclease in ELISA 和 WB. Suitable for Serratia marcescens.
Quick Overview for Nuclease 抗体 (ABIN7540666)
抗原
Nuclease
适用
Serratia marcescens
宿主
兔
克隆类型
多克隆
标记
This Nuclease antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB)
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Supplier Product No.
- 200-401-mu6
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Supplier
- Rockland
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原理
- Nuclease/NucA Antibody
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交叉反应 （详细）
- This antibody will react with endonuclease from S.
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纯化方法
- This antibody was purified from monospecific antiserum by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
- Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:21,600 - 1:22,600
Western_Blot_Dilution: 1:1000
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说明
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Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Nuclease
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别名
- nucA
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背景
- Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA,Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35 °C and 44 °C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.
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UniProt
- P13717
抗原
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