SARS-CoV-2 Nucleocapsid 抗体 (Biotin)
Quick Overview for SARS-CoV-2 Nucleocapsid 抗体 (Biotin) (ABIN7540650)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-406-ms4
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Supplier
- Rockland
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原理
- SARS-CoV-2 Nucleocapsid (N) Protein Antibody Biotin Conjugated
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交叉反应 (详细)
- This affinity purified conjugated antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein.
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纯化方法
- The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 resin.
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免疫原
- Anti-SARS-CoV-2 Nucleocapsid (N) Protein Biotin Conjugated Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:350,000-1:450,000
Western_Blot_Dilution: User Optimized
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说明
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Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody conjugated to biotin has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- SARS-CoV-2 Nucleocapsid
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物质类
- Viral Protein
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背景
- Rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics, and viral research.
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基因ID
- 43740575
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UniProt
- P0DTC9
抗原
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