SARS-CoV-2 Nucleocapsid 抗体
Quick Overview for SARS-CoV-2 Nucleocapsid 抗体 (ABIN7540645)
抗原
See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-ms4s
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Supplier
- Rockland
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原理
- SARS-CoV-2 Nucleocapsid (N) Protein Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein.
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纯化方法
- The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 resin.
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过滤
- Sterile filtered
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免疫原
- Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:500,000 - 1:600,000
Western_Blot_Dilution: User Optimized
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说明
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Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and is suitable in Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).
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: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).
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- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
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别名
- SARS-CoV-2 Nucleocapsid
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物质类
- Viral Protein
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背景
- Rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.
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基因ID
- 43740575
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UniProt
- P0DTC9
抗原
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