MAPK1/2 (pThr202), (pTyr204) 抗体
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Quick Overview for MAPK1/2 (pThr202), (pTyr204) 抗体 (ABIN7540582)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr202, pTyr204
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Supplier Product No.
- 600-401-c76
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Supplier
- Rockland
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原理
- ERK-MAPK phospho T202/phospho Y204 Antibody
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交叉反应 (详细)
- ERK/MAPK pT202/pY204 antibody is directed against human ERK/MAPK.
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纯化方法
- ERK/MAPK pT202/pY204 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.
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免疫原
- Anti-ERK/MAPK Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH.
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亚型
- IgG
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应用备注
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Immunohistochemistry_Dilution: 1:500
Western_Blot_Dilution: 1:1000
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说明
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Anti-ERK/MAPK pT202/pY204 (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42-44 kDa in size corresponding to ERK/MAPK phosphorylated at Thr202 and Tyr204.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- MAPK1/2
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背景
- Mitogen-activated protein kinase 1, MAP kinase 1, MAPK1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42,Anti-ERK/MAPK pT202/pY204 Antibody detects ERK/MAPK which is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204. Anti-ERK/MAPK pT202/pY204 Antibody is ideal for investigators involved in Cell Signaling and Cancer Research.
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基因ID
- 116590
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NCBI登录号
- NP_446294
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UniProt
- P63086
抗原
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