ARNTL 抗体 (N-Term)
Quick Overview for ARNTL 抗体 (N-Term) (ABIN7540572)
抗原
See all ARNTL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-101-ml8s
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Supplier
- Rockland
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原理
- BMAL1 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human Bmal1.
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-BMAL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human Bmal1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:10,000-1:50,000
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: 1:1000
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说明
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Suggested Applications: User Optimized
Anti-BMAL1 Antibody has been tested in WB and IF. Expect a band at ~67-69 kDa in western blot using appropriate lysates. Positive control used: HEK293T and Mouse Brain whole cell lysates in WB, and HeLa cells in IF. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))
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别名
- ARNTL
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背景
- Goat Anti-Aryl hydrocarbon receptor nuclear translocator-like protein 1 Antibody, Goat Anti-Bmal1 Antibody, Arnt3, Brain and muscle ARNT-like 1,Arntl (Aryl Hydrocarbon Receptor Nuclear Translocator Like), also known as Bmal1, is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, Angelman Syndrome and altered circadian rhythm. Anti-Bmal1 Antibody is useful for researchers interested in Neuroscience research.
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基因ID
- 406
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UniProt
- O00327
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途径
- Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effect
抗原
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