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EEF1A2 抗体 (C-Term)

This anti-EEF1A2 antibody is a 山羊 多克隆 antibody detecting EEF1A2 in WB, ELISA, IHC, IF 和 FM. Suitable for 人.
Rockland
产品编号 ABIN7540568
Supplier Product No.: 600-101-ml6s
发货至: 中国

Quick Overview for EEF1A2 抗体 (C-Term) (ABIN7540568)

抗原

See all EEF1A2 抗体
EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

适用

  • 33
  • 16
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1

宿主

  • 37
  • 2
  • 1
山羊

克隆类型

  • 39
  • 1
多克隆

标记

  • 21
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This EEF1A2 antibody is un-conjugated

应用范围

  • 40
  • 18
  • 14
  • 13
  • 13
  • 10
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • 抗原表位

    • 16
    • 8
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Supplier Product No.

    600-101-ml6s

    Supplier

    Rockland

    原理

    EEF1A2 Antibody

    交叉反应 (详细)

    This affinity purified antibody is directed against human EEF1A2.

    纯化方法

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    过滤

    Sterile filtered

    免疫原

    Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).

    亚型

    IgG
  • 应用备注

    ELISA_Dilution: 1:10,000-1:50,000

    Immunohistochemistry_Dilution: 1:200

    IF_Microscopy_Dilution: 15 μg/mL

    Western_Blot_Dilution: 1:1000

    说明

    Anti-EEF1A2 Antibody has been tested in WB, IF, and IHC.  Expect a band at ~50.5 kDa in western blot using appropriate lysates.  Positive control used: human skeletal muscle lysate, MCF-7 nuclear extract lysate in WB, MCF-7 cells in IF, and human skeletal muscle tissue in IHC.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原

    EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

    别名

    EEF1A2

    背景

    Goat Anti-EEF1A2 Antibody, Goat Anti-Eukaryotic Translation Elongation Factor 1 Alpha 2 Antibody, Eukaryotic Elongation Factor 1 A-2, EF-1-Alpha-2, Statin-S1, EEF1AL, STN 3, Elongation Factor 1-Alpha 2, Statin-Like, EEF1A-2, EIEE33, Statin, MRD38, EF1A, STNL, HS1,EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A2 may be associated with the development of ovarian cancer, along with Epileptic Encephalopathy, Early Infantile, 33 and Mental Retardation, Autosomal Dominant 38. Anti-EEF1A2 Antibody is useful for researchers interested in cancer research, translation factors, GTP binding, and GTPase activities.

    基因ID

    1917

    NCBI登录号

    NP_001949

    UniProt

    Q05639
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