EBI3 抗体
Quick Overview for EBI3 抗体 (ABIN7540537)
抗原
See all EBI3 (IL-27b) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 210-301-c55
-
Supplier
- Rockland
-
原理
- IL-35 EBI3 Antibody
-
交叉反应 （详细）
- This antibody is specific for mouse EBI3 protein.
-
纯化方法
- Anti-EBI-3 was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.
-
过滤
- Sterile filtered
-
免疫原
- Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
-
亚型
- IgG2b
-
-
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-229) antibody
IL-27b 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2-3# unconjugated
anti-Interleukin-27 subunit beta (IL-27b) antibodyIL-27b 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-227) antibodyIL-27b 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-229) antibodyIL-27b 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 153-181), (C-Term) antibodyIL-27b 适用: 人 WB 宿主: 兔 Polyclonal RB30602 unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 129-229) antibodyIL-27b 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (C-Term) antibodyIL-27b 适用: 人, 小鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-229) antibodyIL-27b 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 1-1# unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 1-229) antibodyIL-27b 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 129-229) antibodyIL-27b 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1D3 unconjugated
-
-
应用备注
-
Immunoprecipitation_Dilution: 1 μg/mL
ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1 μg/mL
Other: User Optimized
-
说明
-
EBI-3 antibody has been tested for use in ELISA, IP, and Western Blot. Specific conditions for reactivity should be optimized by the end user.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
-
别名
- Ebi3
-
背景
- Mouse anti-EBI3 antibody, mouse anti-IL-35 antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
-
基因ID
- 50498
-
NCBI登录号
- NP_056581
-
UniProt
- O35228
抗原
-
