EBI3 抗体
(2 references)
Quick Overview for EBI3 抗体 (ABIN7540536)
抗原
See all EBI3 (IL-27b) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- dup_210-301-b66s_dup
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Supplier
- Rockland
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原理
- IL-35 EBI3 Antibody
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交叉反应 （详细）
- This antibody is specific for mouse EBI3 protein.
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纯化方法
- Anti-Mouse EBI-3 (MOUSE) Monoclonal Antibody was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
- Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
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亚型
- IgG2b
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anti-Interleukin-27 subunit beta (IL-27b) (AA 21-229) antibody
IL-27b 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2-3# unconjugated
anti-Interleukin-27 subunit beta (IL-27b) antibodyIL-27b 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-227) antibodyIL-27b 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-229) antibodyIL-27b 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 153-181), (C-Term) antibodyIL-27b 适用: 人 WB 宿主: 兔 Polyclonal RB30602 unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 129-229) antibodyIL-27b 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (C-Term) antibodyIL-27b 适用: 人, 小鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 21-229) antibodyIL-27b 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 1-1# unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 1-229) antibodyIL-27b 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin-27 subunit beta (IL-27b) (AA 129-229) antibodyIL-27b 适用: 人 ELISA 宿主: 小鼠 Monoclonal 1D3 unconjugated
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应用备注
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Neutralization_Dilution: 10 μg/mL
Immunoprecipitation_Dilution: 1 μg/mL
ELISA_Dilution: 1:10,000
Western_Blot_Dilution: 1:1000
Other: User Optimized
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说明
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Suggested Applications: Cellular Assay
Anti-Mouse EBI-3 antibody has been tested for use in ELISA and western Blot and is suitable for IP and Neutralization. Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "B lymphocytes ameliorate Alzheimer's disease-like neuropathology via interleukin-35." in: Brain, behavior, and immunity, Vol. 108, pp. 16-31, (2023) (PubMed).
: "The Unknown Aspect of BAFF: Inducing IL-35 Production by a CD5+CD1dhiFcγRIIbhi Regulatory B-Cell Subset in Lupus." in: The Journal of investigative dermatology, Vol. 137, Issue 12, pp. 2532-2543, (2017) (PubMed).
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: "B lymphocytes ameliorate Alzheimer's disease-like neuropathology via interleukin-35." in: Brain, behavior, and immunity, Vol. 108, pp. 16-31, (2023) (PubMed).
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- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
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别名
- Ebi3
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背景
- Mouse anti-EBI3 antibody, mouse anti-IL-35 antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
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基因ID
- 50498
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NCBI登录号
- NP_056581
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UniProt
- O35228
抗原
-
