eEF1A1 抗体 (N-Term)
Quick Overview for eEF1A1 抗体 (N-Term) (ABIN7540533)
抗原
See all eEF1A1 (EEF1A1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 200-901-mp6s
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Supplier
- Rockland
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原理
- EEF1A1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human EEF1A1.
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纯化方法
- This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
- Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 123-462) antibody
EEF1A1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (C-Term) antibodyEEF1A1 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊, 兔, 马, 豚鼠, 山羊 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 1-241) antibodyEEF1A1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 156-255) antibodyEEF1A1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 361-389), (C-Term) antibodyEEF1A1 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19608 unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 131-230) antibodyEEF1A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (acLys41) antibodyEEF1A1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 1-462) antibodyEEF1A1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (AA 1-462) antibodyEEF1A1 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal AT23C11 unconjugated
anti-Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1) (Internal Region) antibodyEEF1A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 5 μg/mL
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说明
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Anti-EEF1A1 Antibody has been tested in Western Blot and IHC. Expect a band at ~48-50 kDa in western blot using appropriate tissues and lysates. Positive control used: MCF-7 nuclear extract lysate in western blot and human kidney tissue in Immunohistochemistry.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
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别名
- EEF1A1
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背景
- Chicken Anti-Eukaryotic Translation Elongation Factor 1 Alpha 1, Leukocyte Receptor Cluster (LRC) Member 7, Leukocyte Receptor Cluster Member 7, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1-Alpha 1, Elongation Factor Tu, EF-1-Alpha-1, EEF1A-1, EEF1A, LENG7, EF-Tu, EF1A, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Translation Elongation Factor 1 Alpha 1-Like 14, Epididymis Secretory Sperm Binding Protein, Elongation Factor 1 Alpha Subunit, Prostate Tumor-Inducing Protein 1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, HNGC:16303, GRAF-1EF, CCS-3, EE1A1, EEF-1, CCS3, PTI1,EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome. Anti-EEF1A1 is useful for researchers interested in Eukaryotic Translation Elongation Factor 1 Alpha-1-Like 14, Cervical Cancer, and the Innate Immune system.
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基因ID
- 1915
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NCBI登录号
- NP_001393
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UniProt
- P68104
抗原
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