eEF1A1 抗体 (N-Term)
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- 抗原 See all eEF1A1 (EEF1A1) 抗体
- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
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抗原表位
- N-Term
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适用
- 人
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This eEF1A1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 200-901-mp6
- Supplier
- Rockland
- 原理
- EEF1A1 Antibody
- 交叉反应 (详细)
- This affinity purified antibody is directed against human EEF1A1.
- 纯化方法
- This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
- Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH).
- 亚型
- IgG
- Top Product
- Discover our top product EEF1A1 Primary Antibody
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- 应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 5 μg/mL
- 说明
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Anti-EEF1A1 Antibody has been tested in Western Blot and IHC. Expect a band at ~48-50 kDa in western blot using appropriate tissues and lysates. Positive control used: MCF-7 nuclear extract lysate in western blot and human kidney tissue in Immunohistochemistry.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))
- 别名
- EEF1A1 (EEF1A1 产品)
- 背景
- Chicken Anti-Eukaryotic Translation Elongation Factor 1 Alpha 1, Leukocyte Receptor Cluster (LRC) Member 7, Leukocyte Receptor Cluster Member 7, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1-Alpha 1, Elongation Factor Tu, EF-1-Alpha-1, EEF1A-1, EEF1A, LENG7, EF-Tu, EF1A, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Translation Elongation Factor 1 Alpha 1-Like 14, Epididymis Secretory Sperm Binding Protein, Elongation Factor 1 Alpha Subunit, Prostate Tumor-Inducing Protein 1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, HNGC:16303, GRAF-1EF, CCS-3, EE1A1, EEF-1, CCS3, PTI1,EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome. Anti-EEF1A1 is useful for researchers interested in Eukaryotic Translation Elongation Factor 1 Alpha-1-Like 14, Cervical Cancer, and the Innate Immune system.
- 基因ID
- 1915
- NCBI登录号
- NP_001393
- UniProt
- P68104
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