EIF4E 抗体 (C-Term)
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- 抗原 See all EIF4E 抗体
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
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抗原表位
- C-Term
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适用
- 人
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This EIF4E antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Supplier Product No.
- 200-901-mk9
- Supplier
- Rockland
- 原理
- EIF4E Antibody
- 交叉反应 (详细)
- This affinity purified antibody is directed against human EIF4E.
- 纯化方法
- This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
- Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-Terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH).
- 亚型
- IgG
- Top Product
- Discover our top product EIF4E Primary Antibody
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- 应用备注
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ELISA_Dilution: User Optimized
Immunohistochemistry_Dilution: 1:1000
Western_Blot_Dilution: 5 μg/mL
- 说明
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Anti-EIF4E Antibody has been tested in Western Blot and IHC. Expect a band at ~25-28kDa in western blot using appropriate tissues and lysates. Positive control used: HEK293T, MOLT-4 and Daudi lysates in Western Blot, Human Lung Adenocarcinoma Tissue in Immunohistochemistry.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
- 别名
- EIF4E (EIF4E 产品)
- 背景
- Chicken Anti-EIF4E Antibody, Eukaryotic Translation Initiation Factor 4E, MRNA Cap-Binding Protein, EIF-4F 25 KDa Subunit, EIF4EL1, EIF-4E, EIF4F, Eukaryotic Translation Initiation Factor 4E-Like 1, AUTS19, EIF4E1, EIF4E, CBP,EIF4E (Eukaryotic Translation Initiation Factor 4E) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. EIF4E is associated with Autism 19 and Pervasive Developmental Disorder. Anti-EIF4E Antibody is useful for researcher interested in cancer research and AKT signaling.
- 基因ID
- 1977
- NCBI登录号
- NP_001124150
- UniProt
- P06730
- 途径
- BCR Signaling
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