SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) 抗体
(2 references)
Quick Overview for SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) 抗体 (ABIN7540513)
抗原
See all SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-a51
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Supplier
- Rockland
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原理
- SARS 3Cl Protease Antibody
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交叉反应 （详细）
- This protein A purified antibody is directed against SARS Coronavirus 3CL Protease.
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纯化方法
- The product was purified from monospecific antiserum by protein A affinity purification.
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免疫原
- This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease. Lifesensors Inc. (www.lifesensors.com) prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.
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亚型
- IgG
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anti-SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8) antibody
SARS-CoV NSP8 适用: SARS Coronavirus-2 (SARS-CoV-2) WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
IF_Microscopy_Dilution: User Optimized
Western_Blot_Dilution: 1:2,000 - 1:10,000
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说明
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This protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome." in: Cell reports, Vol. 37, Issue 4, pp. 109892, (2021) (PubMed).
: "Development of a Cell-Based Luciferase Complementation Assay for Identification of SARS-CoV-2 3CLpro Inhibitors." in: Viruses, Vol. 13, Issue 2, (2021) (PubMed).
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: "Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome." in: Cell reports, Vol. 37, Issue 4, pp. 109892, (2021) (PubMed).
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- SARS-Coronavirus Nonstructural Protein 8 (SARS-CoV NSP8)
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别名
- 3CL-PRO
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物质类
- Viral Protein
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背景
- Rabbit anti-Sars CoV 3CL Protease Antibody, 3CL PRO antibody, 3CLp antibody, nsp5 antibody,Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer. Anti-SARS-CoV 3CL Protease Antibody is useful for researchers interested in viral research.
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基因ID
- 1489680, 29837498
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UniProt
- P0C6U8
抗原
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