5-mC 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in DB, ChIP, ELISA 和 WB. Suitable for 人.
Quick Overview for 5-mC 抗体 (ABIN7540499)
抗原
5-mC
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)
克隆位点
33D3
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Supplier Product No.
- 200-301-v02
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Supplier
- Rockland
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原理
- 5-mC Antibody
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交叉反应 （详细）
- Cross-reactivity with 5-mC from other sources has not been determined.
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纯化方法
- Anti-5-mC Antibody was purified was purified by gel filtration.
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过滤
- Sterile filtered
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免疫原
- Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methylcytosine.
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亚型
- IgG1
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应用备注
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ChIP_Dilution: 1 μg/ChIP
ELISA_Dilution: 1:3,000
Western_Blot_Dilution: 1:1,000
Other: Dot Blot: 1:250
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说明
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Anti-5-mC Antibody is tested for Dot Blotting, ELISA, Immunofluorescence, Methylated DNA Immunoprecipitation (MeDIP), and Sequencing (MeDIP-seq). Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- 5-mC
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背景
- 5-methylcytosine, 5-methyl cytosine,The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. Anti-5mC is ideal for research in Epigenetics due to its applicability to DNA methylation analysis.
抗原
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