5-mC 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in DB, FM, IF 和 IP. Suitable for 人.
Quick Overview for 5-mC 抗体 (ABIN7540498)
抗原
5-mC
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Dot Blot (DB), Fluorescence Microscopy (FM), Immunofluorescence (IF), Immunoprecipitation (IP)
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Supplier Product No.
- 200-301-u98
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Supplier
- Rockland
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原理
- 5-mC Antibody
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交叉反应 （详细）
- Cross-reactivity with 5-mC from other sources has not been determined.
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纯化方法
- Anti-5-mC Antibody was purified by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
- Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methyl Cytidine.
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亚型
- IgG1
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应用备注
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Immunoprecipitation_Dilution: 1-2 μg/IP
IF_Microscopy_Dilution: 1:500
Other: FISH dilution: 1:200 - 1:1,000. Dot Blot: 1:600
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说明
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Suggested Applications: FISH
Anti-5-mC Antibody is tested for IF and Dot Blotting, Fluorescence In Situ Hybridization (FISH), Immunofluorescence and Methylated DNA Immunoprecipitation (MeDIP). Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- 5-mC
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背景
- 5-methylcytidine, 5-methyl Cytidine,5-methyl Cytidine is a modified nucleotide found in the DNA of both plants and vertebrates. Anti-5-mC Antibody is ideal for research in Epigenetics, specifically DNA methylation, due to its applicability to methylation analysis.
抗原
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