Rho-related GTP-binding protein 抗体
Quick Overview for Rho-related GTP-binding protein 抗体 (ABIN7540491)
抗原
See all Rho-related GTP-binding protein (RhO (pan)) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-g36
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Supplier
- Rockland
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原理
- Rho Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Rhodopsin from Mammals, Fish, Avian, Amphibians, and Shark based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Rho Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Rho Antibody was produced in mice by repeated immunizations raised against bovine Rhodopsin.
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亚型
- IgG1
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anti-Rho-related GTP-binding protein (RhO (pan)) antibody
RhO (pan) 适用: 人, 大鼠, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) (C-Term) antibodyRhO (pan) 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) antibodyRhO (pan) 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) antibodyRhO (pan) 适用: Cow WB, IHC 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) antibodyRhO (pan) 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) (Internal Region) antibodyRhO (pan) 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) (AA 62-93) antibodyRhO (pan) 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB52315 unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) antibodyRhO (pan) 适用: 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Rho-related GTP-binding protein (RhO (pan)) (AA 310-339), (C-Term) antibodyRhO (pan) 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal RB36659 unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
ELISA_Dilution: User Optimized
Immunohistochemistry_Dilution: 1:1000
IF_Microscopy_Dilution: User Optimized
Western_Blot_Dilution: 1:1000
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说明
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Anti-Rho Antibody is recommended for use in IF microscopy, IHC, IP, WB, and ELISA. Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
Preservative: 0.09 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Rho-related GTP-binding protein (RhO (pan))
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别名
- RHO
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背景
- OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody, Rhodopsin Antibody,Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chromophore to the surface of the molecule upon light absorption. In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder.
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基因ID
- 509933
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NCBI登录号
- NP_001014890
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UniProt
- P02699
抗原
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