HSP70 1A 抗体
Quick Overview for HSP70 1A 抗体 (ABIN7540482)
抗原
See all HSP70 1A (HSPA1A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-f64
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Supplier
- Rockland
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原理
- Hsp70/Hsc70 Antibody
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交叉反应 （详细）
- A BLAST analysis was used to suggest cross-reactivity with Hsp70/Hsc70 from Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast sources based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-Hsp70/Hsc70 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- Hsp70/Hsc70 Antibody was produced in mice by repeated immunizations raised against chicken Hsp70/Hsp90 complex.
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亚型
- IgG2a
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anti-Heat Shock 70kDa Protein 1A (HSPA1A) (Center) antibody
HSPA1A 适用: 人, 小鼠, 大鼠 WB, IP, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 285-641) antibodyHSPA1A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibodyHSPA1A 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) antibodyHSPA1A 适用: 人, 犬, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3C6 unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 500-600) antibodyHSPA1A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 285-641) antibodyHSPA1A 适用: 人 WB 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (C-Term) antibodyHSPA1A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibodyHSPA1A 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 1-641) antibodyHSPA1A 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal 2# unconjugated
anti-Heat Shock 70kDa Protein 1A (HSPA1A) (AA 418-512) antibodyHSPA1A 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: User Optimized
Immunohistochemistry_Dilution: User Optimized
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-Hsp70/Hsc70 Antibody is tested for WB, IP, IF, and IHC. Expect a band approximately ~72 and ~73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots. This monoclonal antibody reacts with chicken Hsp70/Hsp90 complex. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
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别名
- HSPA1A
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背景
- Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2,Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
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基因ID
- 3303
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NCBI登录号
- NP_005336
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UniProt
- P0DMV8
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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