SOD3 抗体
Quick Overview for SOD3 抗体 (ABIN7540479)
抗原
See all SOD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-f24
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Supplier
- Rockland
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原理
- SOD3 Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with SOD3 from human, rat, and mouse based on 100 % homology with the immunizing sequence.
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纯化方法
- Anti-SOD3 Antibody was purified by Protein G chromatography.
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过滤
- Sterile filtered
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免疫原
- SOD3 Antibody was produced in mice by repeated immunizations with human SOD3 purified from aortas.
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亚型
- IgG1 kappa
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应用备注
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ELISA_Dilution: 1:200
Immunohistochemistry_Dilution: 1:100
IF_Microscopy_Dilution: 1:100
Western_Blot_Dilution: 1:1000
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说明
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Anti-SOD3 Antibody has been tested by WB, IHC and IF and is recommended for use in ELISA. Expect a band approximately ~35kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))
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别名
- SOD3
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背景
- EC-SOD, MGC20077, EC 1.15.1.1, SOD3, Extracellular superoxide dismutase [Cu-Zn],Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. In general, SODs play a major role in antioxidant defense mechanisms. There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.
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基因ID
- 6649
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NCBI登录号
- NP_003093
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UniProt
- P08294
抗原
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