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SOD3 抗体

This anti-SOD3 antibody is a 小鼠 单克隆 antibody detecting SOD3 in WB, IHC, ELISA, IF 和 FM. Suitable for 人.
Rockland
产品编号 ABIN7540479
Supplier Product No.: 200-301-f24
发货至: 中国

Quick Overview for SOD3 抗体 (ABIN7540479)

抗原

See all SOD3 抗体
SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

适用

  • 60
  • 26
  • 10
  • 6

宿主

  • 57
  • 31
  • 3
  • 2
小鼠

克隆类型

  • 62
  • 31
单克隆

标记

  • 62
  • 11
  • 7
  • 3
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  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
This SOD3 antibody is un-conjugated

应用范围

  • 77
  • 58
  • 41
  • 37
  • 22
  • 14
  • 6
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  • 3
  • 3
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

克隆位点

4GG11G6
  • Supplier Product No.

    200-301-f24

    Supplier

    Rockland

    原理

    SOD3 Antibody

    交叉反应 (详细)

    A BLAST analysis was used to suggest cross-reactivity with SOD3 from human, rat, and mouse based on 100 % homology with the immunizing sequence.

    纯化方法

    Anti-SOD3 Antibody was purified by Protein G chromatography.

    过滤

    Sterile filtered

    免疫原

    SOD3 Antibody was produced in mice by repeated immunizations with human SOD3 purified from aortas.

    亚型

    IgG1 kappa
  • 应用备注

    ELISA_Dilution: 1:200

    Immunohistochemistry_Dilution: 1:100

    IF_Microscopy_Dilution: 1:100

    Western_Blot_Dilution: 1:1000

    说明

    Anti-SOD3 Antibody has been tested by WB, IHC and IF and is recommended for use in ELISA. Expect a band approximately ~35kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

    别名

    SOD3

    背景

    EC-SOD, MGC20077, EC 1.15.1.1, SOD3, Extracellular superoxide dismutase [Cu-Zn],Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. In general, SODs play a major role in antioxidant defense mechanisms. There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.

    基因ID

    6649

    NCBI登录号

    NP_003093

    UniProt

    P08294
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