CD65 抗体 (APC)
Quick Overview for CD65 抗体 (APC) (ABIN7540448)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD65 APC
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特异性
- The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
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纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- THP-1 cell line
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亚型
- IgM
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anti-CD65 antibody
适用: 人 FACS 宿主: 小鼠 Monoclonal VIM8 unconjugated
anti-CD65 antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal VIM8 PE
anti-CD65 antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal VIM8 FITC
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- CD65
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背景
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Description: CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.
Other names: ceramide-dodecasaccharide, type II fucoganglioside
抗原
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