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CD65 抗体 (APC)

适用: 人 FACS 宿主: 小鼠 Monoclonal VIM8 APC
产品编号 ABIN7540448
发货至: 中国
  • 抗原
    CD65
    适用
    宿主
    • 3
    小鼠
    克隆类型
    • 3
    单克隆
    标记
    • 1
    • 1
    • 1
    APC
    应用范围
    Flow Cytometry (FACS)
    原理
    Anti-Hu CD65 APC
    特异性
    The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
    纯化方法
    Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    免疫原
    THP-1 cell line
    克隆位点
    VIM8
    亚型
    IgM
  • 应用备注
    Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • 抗原
    CD65
    背景

    Description: CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.

    Other names: ceramide-dodecasaccharide, type II fucoganglioside

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