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PDHA1 抗体 (AA 241-390)

The 小鼠 单克隆 anti-PDHA1 antibody is suitable to detect PDHA1 in samples from 人, 小鼠 和 大鼠. It has been validated for WB, ELISA, IHC, FACS 和 ICC.
产品编号 ABIN7539805
发货至: 中国
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Quick Overview for PDHA1 抗体 (AA 241-390) (ABIN7539805)

抗原

See all PDHA1 抗体
PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

适用

  • 113
  • 77
  • 75
  • 7
  • 7
  • 6
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
人, 小鼠, 大鼠

宿主

  • 110
  • 8
小鼠

克隆类型

  • 83
  • 35
单克隆

标记

  • 41
  • 8
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This PDHA1 antibody is un-conjugated

应用范围

  • 99
  • 35
  • 34
  • 24
  • 19
  • 18
  • 17
  • 13
  • 13
  • 6
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

1G10C10
  • 抗原表位

    • 32
    • 15
    • 7
    • 7
    • 7
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 241-390

    原理

    PDHA1 Antibody

    纯化方法

    Purified antibody

    免疫原

    Purified recombinant fragment of human PDHA1(AA: 241-390) expressed in E. Coli.

    亚型

    IgG1
  • 应用备注

    ELISA: 1/10000

    FCM: 1/200 - 1/400

    ICC: 1/50 - 1/200

    限制

    仅限研究用
  • 缓冲液

    Purified antibody in PBS with 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • 抗原

    PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

    别名

    PDHA1

    背景

    The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

    分子量

    43 kDa

    UniProt

    P08559

    途径

    Warburg Effect
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