DNAJB9 抗体
Quick Overview for DNAJB9 抗体 (ABIN7539265)
抗原
See all DNAJB9 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mdg-1 antibody
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纯化方法
- Protein-A purified
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免疫原
- Recombinant human Mdg-1
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anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (AA 61-75) antibody
Verified DNAJB9 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (AA 114-223) antibodyDNAJB9 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 3G4 unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (AA 24-223) antibodyDNAJB9 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (C-Term) antibodyVerified DNAJB9 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (N-Term) antibodyDNAJB9 适用: 人, 小鼠, 大鼠, 犬, 兔, 豚鼠, 马, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (AA 24-223) antibodyDNAJB9 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (C-Term) antibodyDNAJB9 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (AA 40-69), (N-Term) antibodyDNAJB9 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB33287 unconjugated
anti-Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9) (N-Term) antibodyDNAJB9 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 斑马鱼, 豚鼠, 马, 山羊, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: Use 1-5 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.
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有效期
- 24 months
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- DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))
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别名
- Mdg-1
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背景
- Microvascular endothelial differentiation gene 1 protein, DnaJ homolog subfamily B member 9, ERdj4,Angiogenesis research has focused on receptors and ligands mediating endothelial cell proliferation and migration. Little is known about the molecular mechanisms that are involved in converting endothelial cells from a proliferative to a differentiated state. Microvascular differentiation gene 1 (Mdg1) has been isolated from differentiating microvascular endothelial cells that had been cultured in collagen type I gels (3D culture). In adult human tissue Mdg1 is expressed in endothelial and epithelial cells. Sequence analysis of the full-length cDNA revealed that the N-terminal region of the putative Mdg1-protein exhibits a high sequence similarity to the J-domain of Hsp40 chaperones. It was shown that this region functions as a bona fide J-domain as it can replace the J-domain of Escherichia coli DnaJ-protein. Mdg1 is also upregulated in primary endothelial and mesangial cells when subjected to various stress stimuli. GFP-Mdg1 fusion constructs showed the Mdg1-protein to be localized within the cytoplasm under control conditions. Stress induces the translocation of Mdg1 into the nucleus, where it accumulates in nucleoli. Costaining with Hdj1, Hdj2, Hsp70, and Hsc70 revealed that Mdg1 colocalizes with Hsp70 and Hdj1 in control and stressed HeLa cells. These data suggest that Mdg1 is involved in the control of cell cycle arrest taking place during terminal cell differentiation and under stress conditions.
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基因ID
- 4189
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NCBI登录号
- NM_012328, NP_036460
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UniProt
- Q9UBS3
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途径
- ER-Nucleus Signaling
抗原
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