Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Technical note: When utilised in immunohistochemistry, the use of H2O2 to block endogenous peroxidase has been shown to have a detrimental effect on the epitope recognized. It is, therefore, CRITICAL that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3 % . If these recommendations are not followed, staining intensity will be reduced.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
限制
仅限研究用
状态
Liquid
浓度
29 mg/L
缓冲液
This product is a liquid tissue culture supernatant containing sodium azide as a preservative.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.