Human alpha-sarcoglycan, also known as adhalin. Also crossreacts strongly with alpha-sarcoglycan in sections of muscle from mouse, rat, rabbit, hamster and pig. Does not react with chicken muscle.
产品特性
This product is intended for the qualitative identification by light microscopy of Alpha-Sarcoglycan (Adhalin) by immunohistochemistry.
纯化方法
Tissue culture supernatant
免疫原
Fusion protein containing amino acids 217 to 289 of the rabbit adhalin sequence (Roberds SL et al., 1993).
Immunohistochemistry on frozen sections. Suggested dilution: 1:100-1:200 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
限制
仅限研究用
状态
Liquid
浓度
58.5 mg/L
缓冲液
This product is a liquid tissue culture supernatant containing sodium azide as a preservative.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.