CD52 抗体
Quick Overview for CD52 抗体 (ABIN7538775)
抗原
See all CD52 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kDa.
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交叉反应 （详细）
- No reaction was observed against Human, Mouse and Goat Serum Proteins.
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产品特性
- Purifieded Mouse Monoclonal Anti-Human CD52 is recommended for use in flow cytometry for identification of CAMPATH-1 protein expressed on thymocytes, lymphocytes, monocytes and macrophagues.
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纯化方法
- Purified
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过滤
- 0.22 μm filtered
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免疫原
- Human Peripheral Blood Mononuclear Cells and Tonsil cells
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亚型
- IgG2b kappa
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anti-CD52 (CD52) antibody
CD52 适用: 人, 猕猴, Cynomolgus FACS, WB, IHC (p), ELISA, IHC (fro), CyTox, in vivo 宿主: Human Monoclonal Campath-1H unconjugated Recombinant Antibody
anti-CD52 (CD52) antibody (APC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal HI186 APC
anti-CD52 (CD52) (AA 1-61) antibodyCD52 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD52 (CD52) (AA 20-50) antibodyCD52 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD52 (CD52) antibodyCD52 适用: 人, 猕猴, Cynomolgus FACS, WB, IHC (p), ELISA, IHC (fro), CyTox, in vivo 宿主: 兔 Chimeric Campath-1H unconjugated Recombinant Antibody
anti-CD52 (CD52) antibodyCD52 适用: 人 IHC (f) 宿主: 兔 Monoclonal CD52-2276R unconjugated Recombinant Antibody
anti-CD52 (CD52) antibodyCD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 unconjugated
anti-CD52 (CD52) antibody (FITC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 FITC
anti-CD52 (CD52) antibody (PE)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 PE
anti-CD52 (CD52) antibody (APC)CD52 适用: 人 FACS 宿主: 小鼠 Monoclonal 4C8 APC
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应用备注
- It is recommended for use in flow cytometry. This reagent is effective for indirect immunofluorescence staining of human tissue for flow cytometric analysis using 10 μl/10^6 cells.
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说明
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Purifieded Mouse Monoclonal Anti-Human CD52 is recommended for use in flow cytometry for identification of CAMPATH-1 protein expressed on thymocytes, lymphocytes, monocytes and macrophagues.
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实验流程
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Indirect cell surface Protocol:
- Add 10 µL of CD52 PU to a 12 x 75 mm polystyrene test tube. The 10 µL is a guideline only; the optimal volume should be determined by the individual laboratory
- Transfer 100 µL of anticoagulated (EDTA) blood or the study sample (106 cells) and mix gently with a vortex mixer.
- Incubate in the dark at room temperature (20-25 °C) for 15 minutes or at 4 °C for 30 minutes.
- Add 2 mL 0.01 mol/L PBS and resuspend the cells. Mix well.
- Centrifuge at 540xg for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µL of fluid.
- Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.1 mL PBS
- Add conjugated antibody Anti-Mouse and mix gently with a vortex mixer. Incubate in the dark at room temperature (20-25 °C) for 15 minutes or at 4 °C for 30 minutes.
- Add Lysing Solution according to the manufacturer's directions to each sample and mix gently with a vortex mixer.
- Centrifuge at 540xg for 5 minutes. Gently aspirate the supernatant without disturbing the cell pellet and discard it leaving approximately 50 µL of fluid.
- Add 2 mL 0.01 mol/L PBS (It better that it containing 0,5 % bovine serum albumin) and resuspend the cells. Mix well.
- Centrifuge at 540xg for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µL of fluid.
- Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS + 0,5 % BSA.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.6 mg/mL
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缓冲液
- The conjugate is provided in aqueous buffered solution containing protein stabilizer, and 0.09% sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store in the dark at 2-8 °C.
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有效期
- 12 months
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- CD52
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别名
- CD52
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背景
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CD52 clone HI186 reacts with CD52, also known as CAMPATH-1, a protein of 6,6 kDa. It is expressed on male reproductive tract (epididymis, seminal vesicle), thymocytes, eosinophils, lymphocytes, red cells, monocytes and macrophages, but not on plasma cells, platelets or erythrocytes. CD52 plays a role in carrying and orienting carbohydrate
This molecule is expressed on lymphoid malignancies at variable levels and on epithelial cells of the epididymis and seminal vesicles. Its functional role is not well known but reports indicate that CD52 is a good target for complement-mediated cell lysis and antibody-mediated cellular cytotoxicity. Antibodies to CD52 have been studied in lymphocyte depletion experiments. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Synonyme: CDw52, Cambridge pathology 1 antigen, CAMPATH-1 antigen, Epididymal secretory, protein E5, Human epididymis-specific protein 5, He5
抗原
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