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mCherry 抗体

This anti-mCherry antibody is a 兔多克隆 antibody detecting mCherry in WB, ICC 和 FACS. Suitable for Anaplasma marginale.

Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI, negative control) under reducing and non-reducing conditions. (mCherry 抗体)

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC). (mCherry 抗体)

Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension. (mCherry 抗体)

3 images

产品编号 ABIN7505996

发货至: 中国

Quick Overview for mCherry 抗体 (ABIN7505996)

抗原

mCherry (mCherry Fluorescent Protein)

适用

Anaplasma marginale

宿主

兔

克隆类型

多克隆

标记

17
3
3
1
1
1
1
1
1
1
1
1
1
1
1

This mCherry antibody is un-conjugated

应用范围

32
8
5
4
3
2
1
1
1

Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

原理

Anti-mCherry purified

特异性

The rabbit polyclonal antibody PAb (918) recognizes a red fluorescent protein tag mCherry.

纯化方法

Purified by ligand affinity chromatography.

免疫原

mCherry protein from Anaplasma marginale
anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB, IF, IP, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB 宿主: 小鼠 Monoclonal unconjugated

anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB, IF, ICC 宿主: 小鸡 Polyclonal unconjugated

anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB, IP 宿主: 小鼠 Monoclonal unconjugated

anti-mCherry Fluorescent Protein antibody
适用: Discosoma WB, IF, ICC, IHC 宿主: 小鼠 Monoclonal 1C51 unconjugated

anti-mCherry Fluorescent Protein (AA 1-236) antibody
适用: 标记 WB 宿主: 兔 Polyclonal unconjugated

anti-mCherry Fluorescent Protein antibody
适用: 标记, Drosophila melanogaster WB 宿主: 小鼠 Monoclonal 9F11 unconjugated

anti-mCherry Fluorescent Protein (AA 1-236) antibody (Biotin)
适用: 标记 WB 宿主: 兔 Polyclonal Biotin

anti-mCherry Fluorescent Protein antibody (Biotin)
适用: 标记, Drosophila melanogaster WB 宿主: 小鼠 Monoclonal 9F11 Biotin

应用备注

Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular staining or intracellular staining - depending on expression.
Western blotting: Recommended dilution: 1-2 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
抗原

mCherry (mCherry Fluorescent Protein)

别名

mCherry

背景

The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.

UniProt

X5DSL3

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