CD77 抗体 (PE)
This anti-CD77 antibody is a 大鼠 单克隆 antibody detecting CD77 in FACS. Suitable for 人.
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Quick Overview for CD77 抗体 (PE) (ABIN7505954)
抗原
CD77
适用
人
宿主
大鼠
克隆类型
单克隆
标记
This CD77 antibody is conjugated to PE
应用范围
Flow Cytometry (FACS)
克隆位点
38-13
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原理
- Anti-Hu CD77 PE
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特异性
- The rat monoclonal antibody 38.13 recognizes CD77 (globotriaosylceramide Gb3), a neutral glycosphingolipid expressed mainly on the surface of B cell populations, such as germinal center B cells and Burkitt´s lymphoma cells.
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Daudi cell line (Burkitt´s lymphoma)
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亚型
- IgM
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anti-CD77 antibody
适用: 人 FACS, ICC, IHC (fro) 宿主: 大鼠 Monoclonal 38-13 unconjugated
anti-CD77 antibody (FITC)适用: 人 FACS 宿主: 大鼠 Monoclonal 38-13 FITC
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
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限制
- 仅限研究用
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缓冲液
- Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- CD77
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背景
- CD77 (globotriaosylceramide Gb3), also known as the Pk blood group antigen, BLA (Burkitt´s lymphoma associated antigen), or CTH (ceramide trihexoside) is a neutral glycosphingolipid composed of three carbohydrate molecules linked to a lipid moiety in the cell membrane (Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer). It is expressed on germinal center B cells, Burkitt´s lymphoma cells, it can be induced on extrafolicular B cells and it is also found on endothelia and epithelia. CD77 may be involved in elimination of germinal center B cells that fail to produce high affinity antibodies, and serves also as receptor for shiga toxin and verotoxin.,globotriaosylceramide Gb3, Gal-alpha1-4Gal-beta1-4Glc-beta1-Cer, P blood group
抗原
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