alpha Fetoprotein 抗体 (Biotin)
Quick Overview for alpha Fetoprotein 抗体 (Biotin) (ABIN7505838)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-alpha-Fetoprotein Biotin
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特异性
- The mouse monoclonal antibody AFP-01 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.
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纯化方法
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
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免疫原
- purified alpha-Fetoprotein
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亚型
- IgG1
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anti-alpha-Fetoprotein (AFP) (AA 500-609) antibody
AFP 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 399-605) antibodyAFP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 犬, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 360-609) antibodyAFP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 409-611) antibodyAFP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 小鼠 Monoclonal A2 unconjugated
anti-alpha-Fetoprotein (AFP) (C-Term) antibodyAFP 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 19-397) antibodyAFP 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal OTI2F11 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
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应用备注
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ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).
Western blotting: Recommended dilution: 1-2 μg/mL, non-reducing conditions.
Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- alpha-Fetoprotein
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背景
- Alpha fetoprotein,Alpha-fetoprotein (AFP) is present in fetal plasma, and it binds e.g. copper, nickel, and bilirubin. Measuring of alpha-fetoprotein level in amniotic fluid can reveal severe fetal defects. In adults, elevated AFP concentrations in the plasma can indicate hepatocellular carcinoma or teratoblastoma. In some individuals, hereditary persistance of alpha-fetoprotein can be observed without any obvious pathology.,AFP, FETA, HPAFP
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基因ID
- 174
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UniProt
- P02771
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途径
- C21-Steroid Hormone Metabolic Process
抗原
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