Recombinant T Cell Receptor 抗体
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- 抗原
- T Cell Receptor
- 抗体类型
- Recombinant Antibody
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- Chimeric
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunohistochemistry (IHC)
- 原理
- Anti-TCR [H57-597], Rabbit IgG, lambda
- 特异性
- MAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
- 产品特性
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Original Species of Ab: Hamster, Armenian
Original Format of Ab: IgG
- 纯化方法
- Protein A affinity purified
- 免疫原
- Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
- 克隆位点
- H57-597
- 亚型
- IgG lambda
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anti-T Cell Receptor antibody
适用: 人 FACS, IP, IHC (fro), ELISA 宿主: 小鼠 Monoclonal 11F2 unconjugated
anti-T Cell Receptor antibody适用: 小鼠 FACS, IP, Crys, IF 宿主: 兔 Chimeric Desire-1 unconjugated Recombinant Antibody
anti-T Cell Receptor antibody适用: 小鼠 FACS, IHC 宿主: 仓鼠 Monoclonal GL-3 unconjugated
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- 应用备注
- In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).
- 说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 0.02 % Proclin 300.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- 抗原
- T Cell Receptor
- 别名
- TCR
- 背景
- TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti
- UniProt
- P01852
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