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Recombinant Fc gamma RII (CD32) 抗体

This anti- antibody is a 兔 Chimeric antibody detecting in ELISA, IF, IP, BR 和 InhA. Suitable for 人.
产品编号 ABIN7487694
发货至: 中国

Quick Overview for Recombinant Fc gamma RII (CD32) 抗体 (ABIN7487694)

抗原

See all Fc gamma RII (CD32) 抗体
Fc gamma RII (CD32)

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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Chimeric

标记

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非结合性

应用范围

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ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

克隆位点

IV-3
  • 原理

    Anti-CD32 [IV.3], Rabbit IgG, kappa

    特异性

    This antibody recognizes and binds CD32a.

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2b

    纯化方法

    Protein A affinity purified

    免疫原

    The original antibody was generated against the FcR of K562 cells.

    亚型

    IgG kappa
  • 应用备注

    This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic ""FCGR2A"" mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).

    说明

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    Fc gamma RII (CD32)

    别名

    CD32

    背景

    CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

    UniProt

    P12318
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