Recombinant IL3RA 抗体
Our Local Distributor
北京 101111
Quick Overview for Recombinant IL3RA 抗体 (ABIN7487684)
抗原
See all IL3RA 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-CD123 [7G3], Rabbit IgG, kappa
-
特异性
- This antibody specifically binds to the IL-3R alpha-chain and completely abolishes its function. IL-3R is expressed on myeloid, lymphoid, and vascular endothelial cells, where it transduces IL-3-dependent signals leading to cell activation.
-
产品特性
-
Original Species of Ab: Mouse
Original Format of Ab: IgG2a
-
纯化方法
- Protein A affinity purified
-
免疫原
- The original antibody was generated by intraperitoneally immunizing BALB/c mice with 5 X lo6 COS cells transfected with the IL-3R a-chain in combination with adjuvant peptides.
-
亚型
- IgG kappa
-
-
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 200-305) antibody
IL3RA 适用: 人 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E11C5 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibody (PE)IL3RA 适用: 人 FACS 宿主: 小鼠 Monoclonal 6H6 PE
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-158) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 201-300) antibodyIL3RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 167-331) antibodyIL3RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 1-100) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 26-171) antibodyIL3RA 适用: 人 IHC, ELISA, Coat, ISt, StM 宿主: 兔 Monoclonal IL3RA-2947R unconjugated Recombinant Antibody
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-305) antibodyIL3RA 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM31 unconjugated
-
-
应用备注
- This antagonist antibody binds IL-3Rα and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R α chain expressed by transfected cells and bound to primary cells expressing IL-3Rα. The expression of IL-3Rα chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Rα and βc dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38- cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcγRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).
-
说明
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- PBS with 0.02 % Proclin 300.
-
储存液
- ProClin
-
注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
-
- IL3RA (Interleukin 3 Receptor, alpha (IL3RA))
-
别名
- CD123
-
背景
- IL3RA, IL-3RA, IL-3R-alpha, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha
-
UniProt
- P26951
-
途径
- JAK/STAT Signaling
抗原
-
