Fibronectin 抗体
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Quick Overview for Fibronectin 抗体 (ABIN7478856)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Mouse fibronectin
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交叉反应 (详细)
- Mouse
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产品特性
- This antibody may be used to identify mouse fibronectin in experimental studies on wound healing, tumour progression, and tumour invasion, etc. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.
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纯化方法
- Protein G affinity purification
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免疫原
- Purified fibronectin from murine plasma
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应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin,IFA, Immunohistochemistry on frozen and paraffin sections, ELISA, RIA,IHC 60 min at RT or 2-8 °C over night,IHC (frozen): 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.
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限制
- 仅限研究用
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浓度
- 5 mg/mL
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缓冲液
- Affinity purified (ProtG) antiserum lyophilized from phosphate buffered solution (non-sterile). Contains no BSA or sodium azide! Reconstitute with 0.5 mL of sterile water and store aliquots at -20 °C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- -20°C
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- Fibronectin
抗原
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