COL3 抗体
Quick Overview for COL3 抗体 (ABIN7478855)
抗原
See all COL3 抗体适用
宿主
克隆类型
标记
应用范围
-
-
交叉反应
- 小鼠
-
交叉反应 （详细）
- Rat, cross-reaction with mouse
-
产品特性
- Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type III 100 % , rat collagen I <0.1 %, rat collagen type V <10 %, rat elastin <0.1 % (solid phase RIA at 1:50 dilution).
-
纯化方法
- Affinity purified antibody
-
免疫原
- Purified collagen type III from rat skin
-
-
anti-Collagen, Type III (COL3) (AA 1301-1400) antibody
COL3 适用: 人, 大鼠, 小鼠, 兔, 犬 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) (AA 801-900) antibodyCOL3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type III (COL3) antibody (Biotin)COL3 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人, 大鼠, 小鼠 WB 宿主: 小鼠 Monoclonal Q76 unconjugated
anti-Collagen, Type III (COL3) antibody (Magnetic Particles)COL3 适用: 人, 大鼠, 小鼠 IP 宿主: 小鼠 Monoclonal Col-29 Magnetic Particles
anti-Collagen, Type III (COL3) (AA 81-130) antibodyCOL3 适用: 人, 大鼠, 小鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Rat skin or liver,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
-
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Lyophilized from phosphate buffered solution, no BSA and preservative added!
-
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- -20°C
-
-
- COL3 (Collagen, Type III (COL3))
-
别名
- Collagen Type III
抗原
-
