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COL3 抗体

This 兔 多克隆 antibody specifically detects COL3 in WB, ELISA, IF, IHC (p) 和 RIA. It exhibits reactivity toward 小鸡.
产品编号 ABIN7478854
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for COL3 抗体 (ABIN7478854)

抗原

See all COL3 抗体
COL3 (Collagen, Type III (COL3))

适用

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  • 1
小鸡

宿主

  • 55
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克隆类型

  • 53
  • 18
多克隆

标记

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This COL3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
  • 交叉反应

    Cow, 人, 小鼠, 大鼠

    交叉反应 (详细)

    Chicken, cross-reaction with human, bovine, mouse and rat collagen type III <0,1 %

    产品特性

    Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type III 100 % , chicken collagen type I <5 %.

    纯化方法

    Affinity purified antibody

    免疫原

    Purified collagen type III from chicken skin
  • 应用备注

    After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Chicken skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:500, ELISA > 1:200

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Lyophilized from phosphate buffered solution, no BSA and preservative added!

    储存液

    Without preservative

    储存条件

    -20 °C

    储存方法

    -20°C
  • 抗原

    COL3 (Collagen, Type III (COL3))

    别名

    Collagen Type III
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