Vaccinia Virus 抗体 (Peroxidase (POD))
This anti-Vaccinia Virus antibody is a 兔 多克隆 antibody detecting Vaccinia Virus in IHC, WB 和 EIA. Suitable for Vaccinia Virus (VACV).
Quick Overview for Vaccinia Virus 抗体 (Peroxidase (POD)) (ABIN7478353)
抗原
Vaccinia Virus (VACV)
(Vaccina Virus (VACV))
适用
Vaccinia Virus (VACV)
宿主
兔
克隆类型
多克隆
标记
This Vaccinia Virus antibody is conjugated to Peroxidase (POD)
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
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特异性
- Purified virions
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交叉反应 （详细）
- Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox
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纯化方法
- This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
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免疫原
- New York City Board of Health (NYCBOH) strain
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亚型
- IgG
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应用备注
- TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Vaccinia Virus (VACV) (Vaccina Virus (VACV))
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别名
- Vaccina Virus
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物质类
- Virus
抗原
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