Reactive with proteins from common Clostridia species
交叉反应 (详细)
Antiserum is not absorbed & may react with related microorganisms, Reactive (IFA) with Cl. perfingens, Cl. sordellii, Cl. novyi, Cl. septicum, Cl. chauvoei
纯化方法
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
TITER : >1:500 by indirect IFA, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.