Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
特异性
AA 546-682 sequence from strain HxB2
交叉反应 (详细)
Antiserum does not cross-react with human T or B cells
纯化方法
This product consists of purified IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
免疫原
recombinant (P. Pastoris) ectodomain of gp41 (glycosylated)
HIV-1 gp41
适用: Human Immunodeficiency Virus (HIV)
宿主: 兔
Polyclonal
unconjugated
应用备注
TITER : > 1:100 by western blot immunoassay, Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1 % sodium azide preservative. No stabilizing proteins have been added.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.