Quick Overview for Human Adenovirus Hexon (HAdV Hexon) 抗体 (Peroxidase (POD)) (ABIN7478201)
抗原
Human Adenovirus Hexon (HAdV Hexon)
适用
Adenovirus
宿主
山羊
克隆类型
多克隆
标记
Peroxidase (POD)
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)
特异性
Numerous Adeno proteins including hexon
交叉反应 (详细)
Reactive with Adeno types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Others not tested. Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2, LLCMK2 & Vero cells by indirect immunofluorescence
纯化方法
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
免疫原
gradient purified, disrupted Adenovirus type 5 virions
TITER : > 1: 5,000 by ELISA, > 1:8,000 in IFA, > 1:1,000 in western blot immunoassay, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
储存液
Thimerosal (Merthiolate)
注意事项
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.