Quick Overview for Human Adenovirus Hexon (HAdV Hexon) 抗体 (FITC) (ABIN7478200)
抗原
Human Adenovirus Hexon (HAdV Hexon)
适用
Adenovirus
宿主
山羊
克隆类型
多克隆
标记
FITC
应用范围
Functional Studies (Func)
特异性
Numerous Adeno proteins including hexon
交叉反应 (详细)
Reactive with Adeno types 1, 2, 3, 5, 6, 7a, 8, 31, 40 & 41. Others not tested. Does not crossreact with Para 1-3, Infl. A & B, RSV, hMPV & Rhinovirus (type 16) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2, LLCMK2 & Vero cells by indirect immunofluorescence
纯化方法
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
免疫原
gradient purified, disrupted Adenovirus type 5 virions
TITER : > 1: 5,000 by ELISA, > 1:8,000 in IFA, > 1:1,000 in western blot immunoassay, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
限制
仅限研究用
状态
Liquid
浓度
Lot specific
缓冲液
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.