E. Coli 抗体 (Peroxidase (POD))
This Peroxidase (POD)-conjugated 山羊 多克隆 anti-E. Coli antibody specifically detects E. Coli in EIA, WB 和 IHC. The antibody is reactive with E. coli samples.
产品编号 ABIN7478178
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for E. Coli 抗体 (Peroxidase (POD)) (ABIN7478178)
抗原
E. Coli
适用
E. coli
宿主
山羊
克隆类型
多克隆
标记
This E. Coli antibody is conjugated to Peroxidase (POD)
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)
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特异性
- Many "O" and "K" antigenic serotypes of Escherichia coli
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交叉反应 （详细）
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
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纯化方法
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
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免疫原
- Mixture of E. coli serotypes
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亚型
- IgG
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应用备注
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TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- E. Coli
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物质类
- Bacteria
抗原
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